Magnetic mesoporous silica/graphene oxide based molecularly imprinted polymers for fast selective separation of bovine hemoglobin

In this work, a facile protein imprinted polymer based on magnetic rich-amine mesoporous silica/graphene oxide was synthesized using dopamine as the functional monomer and bovine hemoglobin as the template molecule. Fe3O4 nanoparticles were introduced onto graphene oxide by an inverse microemulsion method for facilitating surface polymerization dopamine imprinted layer. The protein adsorption test showed that the imprinted layer based on magnetic rich-amine mesoporous silica/graphene oxide can significantly shorten the adsorption equilibrium time of the imprinted polymer toward bovine hemoglobin. Under optimized conditions, the imprinting factor and maximum adsorption capacity of the magnetic imprinted polymer toward bovine hemoglobin was 2.09 and 164.5 mg g(-1), respectively. Several experimental parameters including temperature and pH of solution, type and volume of elution solvent, washing solvent, extraction time were investigated in detail. Combined with magnetic solid phase extraction and high performance liquid chromatography, the magnetic imprinted polymers was successfully applied to rapid separate and enrich bovine hemoglobin from bovine serum with the recoveries of 84.6-102.5%.