Roseitalea porphyridii gen. nov., sp nov., isolated from a red alga, and reclassification of Hoeflea suaedae Chung et al. 2013 as Pseudohoeflea suaedae gen. nov., comb. nov

A Gram-staining-negative, strictly aerobic bacterial strain, designated MA7-20(T), was isolated from a marine alga, Porphyridium marinum, in Korea. Cells showing oxidase-positive and catalase-positive activities were motile rods with bipolar flagella. Growth of strain MA7-20(T) was observed at 15-45 C-circle (optimum, 30-37 C-circle), at pH 6.0-10.5 (optimum, pH 7.0-8.0) and in the presence of 0-7% (w/v) NaCl (optimum, 2-3 %). Strain MA7-20(T) contained summed feature 8 (comprising C-18 : 1 omega 7c/ C-18 : 1 omega 6c), 11-methyl C-18 : 1 omega 7c and C-18 : 0 as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidyl-N-methylethanolamine. The G+C content of the genomic DNA was 61.5 mol%. Strain MA7-20(T) was most closely related to Hoeflea suaedae YC6898(1) , Oricola cellulosilytica CC-AMH-0(1) and Nitratireductor basaltis J3(1)with 96.0, 95.8 and 95.8% 16S rRNA gene sequence similarities, respectively, but the strain formed a distinct phylogenetic lineage from them within the family Phyllobacteriaceae with a low bootstrap value. H. suaedae also formed a clearly distinct phylogenetic lineage from other members of the genus Hoeflea and closely related genera. On the basis of phenotypic, chemotaxonomic and molecular properties, strain MA7-20(T) represents a novel species of a new genus of the family Phyllobacteriaceae, for which the name Roseitalea porphyridii gen. nov., sp. nov. is proposed. The type strain is MA7-20(T) (=KACC 18807(T)=JCM 31538(T)). In addition, H. suaedae is also reclassified as Pseudohoeflea suaedae gen. nov., comb. nov. (type strain YC6898(T)=KACC 14911(T)=NBRC 107700(T)).