Paenibacillus apis sp nov and Paenibacillus intestini sp nov., isolated from the intestine of the honey bee Apis mellifera

Three strains, CBM1(T), CBH23 and LAH16(T), belonging to the genus Paenibacillus were isolated from the midgut and hindgut of Apis mellifera. Based on 16S rRNA gene sequence analyses and phenotypic characteristics, the three strains represent two novel species. Strains CBM1 T and CBH23 formed a group with Paenibacillus puldeungensis CAU 9324(T), and strain LAH16 T belonged to the Paenibacillus amylolyticus NRRL NRS-290 T subgroup of the genus Paenibacillus. The DNA G+ C contents of strains CBM1(T), CBH23 and LAH16 T were 47.7, 48.1 and 46.1 mol%, respectively. The three strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as polar lipids. The predominant quinone in the three strains was MK-7, but strains CBM1 T and CBH23 contained an additional major quinone, MK-8(H-2). While strain LAH16 T and most species of the genus Paenibacillus possessed anteiso-C-15 : 0, iso-C-16 : 0 and C-16 : 0 as major cellular fatty acids, strains CBM1(T) and CBH23 possessed C-16 : 0, anteiso-C-15 : 0, C-18 : 1 omega 7c and C19 : 0cyclo omega 8c. Based on phenotypic, chemotaxonomic and phylogenetic data, strains CBM1(T) and CBH23 and the strain LAH16(T) represent novel species in the genus Paenibacillus, for which the names Paenibacillus apis sp. nov. and Paenibacillus intestini sp. nov. are proposed, with CBM1(T) (= KCTC 33844(T) = JCM 31620(T)) and LAH16(T) (= KCTC 33832(T) = JCM 31621(T)) as the type strains, respectively.