期刊
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
卷 67, 期 7, 页码 2430-2435出版社
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.001977
关键词
New taxa; Clostridium cluster XIVa; human gut microbiota
类别
资金
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) [0534-20150013]
- National Research Foundation of Korea (NRF) [NRF-2015R1A2A1A10054078]
- Global PhD Fellowship program [NRF-2012H1A2A1049234]
A strictly anaerobic Gram-stain-positive, non-motile and non-spore-forming bacterial strain, BR31(T), was isolated from a faecal sample of a healthy human. Bacterial colonies were ivory-coloured on GAM agar and composed of rod-shaped cells with rounded ends approximately 1.4-2.1 x 0.5-0.6 mu m in size. According to comparative analysis based on 16S rRNA gene sequences, strain BR31 T formed a distinct phylogenetic lineage that reflects a new genus within Clostridium cluster XIVa in the family Lachnospiraceae, with highest similarity to Eubacterium contortum DSM 3982(T) (94.6 %). The DNA G+C content was calculated to be 47.0 mol% from whole genome sequencing. Predicted genes associated with synthesis of teichuronic acid, polyamines, polar lipids and diaminopimelic acid were detected. The peptidoglycan contained meso-diaminopimelic acid and the main polar lipid detected was phosphatidylglycerol. The major metabolic end product of glucose was acetic acid, which was in agreement with those of most members of the family. However, the profile of major cellular fatty acids (C-16 : 0, C-14 : 0, summed feature 4 and C-13 : 0) and overall enzyme activity demonstrated phenotypic differentiation of strain BR31(T) from other closely related genera. Thus, based on distinct phenotypic, phylogenetic, genotypic and chemotaxonomic characteristics, strain BR31(T) is considered to represent a novel species of a new genus, for which the name Merdimonas faecis gen. nov., sp. nov. is proposed. The type strain of Merdimonas faecis is BR31(T) (=KCTC 15482(T)=JCM 30748(T)).
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