Bacillus solisilvae sp nov., isolated from forest soil

A novel Gram-stain-positive, motile, endospore-forming, rod-shaped bacterial strain, NEAU-cbsb5(T), was isolated from forest soil from Changbai Mountain, Heilongjiang Province, China. The isolate grew at 15-40 degrees C (optimum 30 degrees C), at pH 6.0-8.0 (optimum pH 7.0) and in the presence of up to 4% (w/v) NaCl, although NaCl was not required for growth. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-cbsb5(T) formed a distinct lineage within the genus Bacillus and was most closely related to Bacillus acidiceler DSM 18954(T) (99.1% similarity) and Bacillus luciferensis JCM 12212(T) (99.0 %). 16S rRNA gene sequence similarity to sequences of the type strains of other Bacillus species was less than 96.0 %. Average nucleotide identity (ANI) values between NEAU-cbsb5(T) and its most closely related species were 78.72-84.75% by ANIm, ANIb and OrthoANIu analysis. The in silico DNA-DNA hybridization values between strain NEAU-cbsb5(T) and its close relatives B. acidiceler DSM 18954(T) and B. luciferensis JCM 12212(T) were both 23.80 %, again indicating they belong to different taxa. The major cellular fatty acids of NEAU-cbsb5(T) were iso-C-15 : 0, anteiso-C-15 : 0 and C-16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unknown aminophospholipid. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinones were MK-7 and MK-6. The genomic DNA G+C content was 33.0 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic data, strain NEAU-cbsb5(T) was classified as a representative of a novel species in the genus Bacillus, for which the name Bacillus solisilvae sp. nov. is proposed. The type strain is NEAU-cbsb5(T) (=CGMCC 1.14993(T) =DSM 100485(T)).