Leucobacter weissii sp nov., an isolate from activated sludge once described as first representative of the peptidoglycan variation B2δ, and emended description of the genus Leucobacter

Strain S27(T) is a Gram-stain-positive, regular rod-shaped, non-motile, non-spore-forming, yellow pigmented actinobacterium which was isolated from an aerated laboratory scale fermenter fed with wastes of a yeast factory. The strain was classified as Microbacterium sp. after the analysis of its peptidoglycan revealed a novel B-type structure established as variation B2 delta by Hensel in 1984. As the combination of the peptidoglycan amino acids 2,4-diaminobutyric acid (Dab), threonine (Thr), glycine (Gly), alanine (Ala) and glutamic acid (Glu) is in disagreement with the current genus definition of Microbacterium but is typical of several Leucobacter species, the taxonomic status of strain S27(T) was re-examined by a polyphasic study. Comparative analyses of 16S rRNA gene sequences and the occurrence of L-Dab, D-Ala, L-Ala, Gly, L-Thr, D-Glu and lower amounts of L-Glu in the peptidoglycan in combination with the predominating menaquinones MK-11, MK-10 and MK-9, phosphatidylglycerol, and one unknown glycolipid as the major polar lipids (and trace amounts of diphosphatidylglycerol and an unknown phospholipid), a profile with anteiso-C-15 : 0, iso-C-15 : 0, iso-C-16 : 0, anteiso-C-17 : 0 and iso-C-17 : 0 as major fatty acids and the G+ C value of 70.1 mol% confirmed the affiliation to the genus Leucobacter and revealed that S27(T) (= DSM 20621(T) = CCM 8762(T)) is the type strain of a new species for which the name Leucobacter weissii sp. nov. is proposed. The availability of new data allows for an emended description of the genus Leucobacter.