Neisseria dumasiana sp nov from human sputum and a dog's mouth

Three independent isolates of Gram-reaction-negative cocci collected from two New York State patients and a dog's mouth in California were subjected to a polyphasic analysis. The 16S rRNA gene sequence similarity among these isolates is 99.66 to 99.86 %. The closest species with a validly published name is Neisseria zoodegmatis (98.7% 16S rRNA gene sequence similarity) with six additional species of the genus Neisseria with greater than 97% similarity. Average nucleotide identity (ANI) and genome-to-genome distance calculator (GGDC 2.0) analysis on whole genome sequence data support the three novel isolates as being from a single species that is distinct from all other closely related species of the genus Neisseria. Phylogenetic analysis of 16S rRNA gene sequences and ribosomal multilocus sequence typing (rMLST) indicate the novel species belongs in the genus Neisseria. This assignment is further supported by the predominant cellular fatty acids composition of C-16 : 0, summed feature 3 (C-16 : 1 omega 7c/C(15 : 0)iso 2-OH), and C-18 : 1 omega 7c, and phenotypic characters. The name Neisseria dumasiana sp. nov. is proposed, and the type strain is 93087(T) (= DSM 104677(T) = LMG 30012 (T)).