期刊
STAR PROTOCOLS
卷 1, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2020.100041
关键词
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资金
- Comprehensive Heart Failure Center Wurzburg (CHFC Wurzburg)
- German Research Foundation (DFG) [SFB688 TP A19, TR225-B04]
- IZKF-Wurzburg (Interdisziplinares Zentrum fur Klinische Forschung der Universitat Wurzburg)
- University of Wurzburg
Organoids are three-dimensional (3D) constructs generated in stem cell cultures and are thought to mimic tissue and organ development in situ. However, until recently, they often exclusively recapitulated the development of the organ's parenchyma without the major components of the organ stroma. Here, we describe a protocol to incorporate stromal components, first of all blood vessels, by co-culturing with induced pluripotent stem cell-derived mesodermal progenitor cells. For complete details on the use and execution of this protocol, please refer to Worsdorfer et al. (2019).
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