期刊
BLOOD SCIENCE
卷 2, 期 3, 页码 79-88出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/BS9.0000000000000049
关键词
Induced hematopoietic progenitor cells; Pluripotent stem cells; T cells regeneration; Transplantation
类别
资金
- Strategic Priority Research Program of Chinese Academy of Sciences [XDA16010601]
- Health and Medical Care Collaborative Innovation Program of Guangzhou Scientific and Technology [201803040017]
- CAS Key Research Program of Frontier Sciences [QYZDB-SSW-SMC057]
- National Key R&D Program of China [2019YFA0110200]
- Major Research and Development Project of Guangzhou Regenerative Medicine and Health Guangdong Laboratory [2018GZR110104006]
- Science and Technology Planning Project of Guangdong Province [2017B030314056]
- National Natural Science Foundation of China [81925002]
Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells using an inducible Runx1-Hoxa9-PSC (iR9-PSCs) line. The procedure mainly includes generation of induced hematopoietic progenitor cells (iHPCs) in vitro, transplantation, and development of functional induced T cells (iT) in vivo via transplantation. The entire induction process in vitro requires 21 days before iHPCs transplantation. The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation. We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose.
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