期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 532, 期 1, 页码 647-655出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2017.08.074
关键词
Targeted Drug Delivery; Nanomedicine; Polymeric Micelles; Scavenger Receptors Class A; Angiogenesis; Oligonucleotides
资金
- Faculty of Medicine, The Chinese University of Hong Kong [4054295]
- Faculty of Medicine, The Chinese University of Hong Kong
- Chinese University of Hong Kong
Spherical nucleic acid gold nanoparticles represent a unique nanotechnology in which the spherical arrangement of oligonucleotides enables the nanoparticles to be efficiently internalized into cells expressing scavenger receptors class A (SR-A). Herein, we seek to replace the gold core with a biodegradable polymeric construct and explore their potential applications in targeted drug delivery. Oligonucleotide-conjugated poly(ethylene glycol)-block-poly(epsilon-caprolactone) was synthesized and characterized by H-1 NMR and gel electrophoresis. This polymer was applied to fabricate micellar nanoparticles (OLNNPs) by an anti-solvent method. These nanoparticles have a mean particle size about 58.1 nm with a narrow size distribution (PDI < 0.2) and they were also non-cytotoxic. Relative to non-targeted NPs, OLN-NPs exhibited substantially better uptake (3.94 x) in a mouse endothelial cell line (C166), attributing to lipid-raft-mediated endocytosis via SR-A. To explore the potential applications of OLN-NPs as drug carriers, paclitaxel, a poorly soluble anti-angiogenic compound, was selected as the model. OLN-NPs increased the solubility of paclitaxel by at least 300 x. The boosted drug solubility in conjunction with improved cellular uptake translated into enhanced in vitro efficacy in the inhibition of angiogenesis. In conclusions, OLN-NPs show considerable promise in targeted drug delivery and their potential applications should be further investigated.
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