3.9 Article

Sustained Release of Catechin from Gelatin and Its Effect on Bone Formation in Critical Sized Defects in Rat Calvaria

期刊

JOURNAL OF HARD TISSUE BIOLOGY
卷 29, 期 2, 页码 77-84

出版社

JOURNAL HARD TISSUE BIOLOGY
DOI: 10.2485/jhtb.29.77

关键词

Bone formation; EGCG; Catechin; Gelatin

资金

  1. Japan Society for the Promotion of Science [16K11667, 18H02986]
  2. Grants-in-Aid for Scientific Research [18H02986, 16K11667] Funding Source: KAKEN

向作者/读者索取更多资源

The potential of plant-derived polyphenols in bone tissue engineering has not been fully realized owing to difficulties in maintaining their stability in affected parts. Catechins, such as epigallocatechin gallate (EGCG), are not fully utilized in bone regenerative medicine. Here, we demonstrated that chemical and non-chemical modifications of gelatin with EGCG resulted in distinct bone-forming abilities in critical-sized bone defects (9 mm) in rat calvaria. We prepared two EGCG-containing gelatin sponges: vacuum-heated gelatin sponges modified chemically with EGCG (AC-vhEGCG-GS) and vacuum-heated gelatin sponges with EGCG (no chemical modification; NC-vhEGCG-GS). Both sponges were characterized using scanning electron microscopy and degradability and EGCG-retention tests. The bone-forming ability of the sponges were estimated using micro-computed tomography and hematoxylin-eosin staining; the quality of newly formed bone (collagen maturation) was determined using picrosirius red staining and polarized microscopy. Both sponges had a spongy and soft texture with macropores ranging 50-150 mu m with negligible differences in degradability. The NC-vhEGCG-GSs released all their EGCG content within 1 h, whereas AC-vhEGCG-GSs retained 75% of the EGCG for up to 24 h. In addition, AC-vhEGCG-GSs resulted in a significantly greater bone formation than NC-vhEGCG-GSs 4 w after implantation, with negligible differences in collagen maturation. These results suggest that the chemical modification of gelatin with EGCG might be a promising strategy to fully utilize the pharmacological effects of EGCG for natural polymer-based sponges. Moreover, the release rate of EGCG from gelatin is possibly a screening parameter affecting the function of EGCG in vivo.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

3.9
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据