期刊
INTERNATIONAL JOURNAL OF PEST MANAGEMENT
卷 64, 期 1, 页码 66-76出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09670874.2017.1327681
关键词
Aflatoxin; ELISA; fumonisin; mycotoxins; sorghum
类别
资金
- Ethiopian Ministry of Education - Haramaya University
- Wolaita Sodo University
- German Academic Exchange Service (DAAD)
Sanitation from harvest to storage is a key factor in eliminating sources of infection and reducing levels of mycotoxigenic fungal invasion, and thereby mycotoxin contamination. This study was conducted at Haramaya University, Eastern Ethiopia to investigate the effects of threshing methods on fungal invasion and mycotoxin contamination of sorghum grain. The methods included threshing on bare ground, cow-dung-painted ground, concrete asphalt and canvas. The threshed grain was kept separately in bags in three replications in a completely randomized design and stored for five months. The results revealed that all the sorghum grain samples taken from different threshing methods were contaminated with both Aspergillus and Fusarium species. There were variations in fungal invasion between samples taken immediately after threshing and stored samples. The level of Aspergillus spp. invasion was much higher in the latter in sorghum grain threshed on bare ground. The concentration of aflatoxin B-1 was gradually increased, whereas the total fumonisin concentration was decreased with the storage duration both in 2013 and 2014. On stored sorghum grain, the highest (1.97 mu g kg(-1)) and the lowest (0.70 mu g kg(-1)) mean aflatoxin B1 concentrations were recorded from sorghum grains threshed on bare ground and on canvas, respectively. Sorghum grain obtained from canvas threshing method had significantly lower mean total fumonisin content (142.5, 54.9 mu g kg(-1)) than grain from the other methods at threshing. The current work clearly demonstrated the effect that threshing might have on fungal invasion and mycotoxin contamination of sorghum grains.
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