期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 51, 期 6, 页码 1909-1919出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2017.4149
关键词
di-2-pyridylhydrazone dithiocarbamate s-acetic acid; antiproliferative activity; apoptosis; p53; autophagy; antagonistic effect; copper complex; ROS; mechanism
类别
资金
- Natural Science Foundation of China [21571153]
- Henan Science and Technology Agency [114300510012, 132102310250, 152300410118]
- Plan of Health Scientific and Technological Innovation Talents of Henan Province [2109901]
- Graduate innovation project of Xinxiang Medical University [YJSCX201613Y]
Cancer cells have higher demand of iron and copper ions for growth, disturbing the metal's homeostasis can inhibit proliferation of cancer cell. Dithiocarbamates possessing excellent metal chelating ability and antitumor activity are considered as candidates in chelation therapy, however, their antitumor molecular mechanisms remain to be elucidated. In the present study, a dithiocarbamate derivative, di-2-pyridylhydrazone dithiocarbamate s-acetic acid (DpdtaA) was prepared to address the issue whether the molecular mechanism behind biological behavior showed by dithiocarbamate was p53 mediated. The proliferation inhibition assay showed that DpdtaA exhibited excellent antiproliferative effect for hepatocellular carcinoma ( IC50= 3.0 +/- 0.4 mu M for HepG2, 6.1 +/- 0.6 mu M for Bel-7402 cell). However, in the presence of copper ion, the antiproliferative activity of DpdtaA significantly attenuated (similar to 3-fold for HepG2) due to formation of copper chelate. The ROS assay revealed that the antiproliferative activity of DpdtaA correlated with ROS generation. Western blotting demonstrated that DpdtaA could upregulate p53 via downregulating the Mdm2, accordingly leading to changes of bcl family proteins, indicating that a p53-dependent intrinsic apoptosis was partly involved. Simulation from molecular docking hinted that DpdtaA could disrupt interaction between p53 and Mdm2, indicating the disruption might also contribute to the upregulation of p53. The alternations in lysosome membrane permeability and acidic vacuoles as well as LC3-II upregulation indicated that autophagy was involved. The copper addition led to significantly attenuate biological activity of DpdtaA, with few dithiocarbamates, but the mechanism in apoptosis induction was not altered except for weaker ability.
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