期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 51, 期 5, 页码 1405-1414出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2017.4123
关键词
human amniotic epithelial cell; epithelial ovarian cancer; cell cycle; TGF-beta 1
类别
资金
- National Natural Science Foundation of China [81370678]
- Shanghai Municipal Council for Science and Technology [14411961500]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine [20152236]
- Shanghai Municipal Health Bureau [Y20140241]
- Shanghai Jiao Tong University Medicine-Engineering Fund [YG 2014QN12]
It is reported that human amniotic epithelial cells (hAECs) endow intrinsic antitumor effects on certain kinds of cancer. This research was designed to evaluate whether hAECs endowed potential anticancer properties on epithelial ovarian cancer (EOC) cells in vivo and in vitro, which has not been reported before. In this study, we established a xenografted BALB/c nude mouse model by subcutaneously co-injecting ovarian cancer cell line, SK-OV-3, and hAECs for 28 days. In ex vivo experiments, CCK-8 cell viability assay, real-time PCR, cell counting assay, cell cycle analysis and immunohistochemistry (IHC) assay were used to detect the effects of hAEC-secreted factors on the proliferation and cell cycle progression of EOC cells. A cytokine array was conducted to detect anticancer-related cytokines released from hAECs. Human recombinant TGF-beta 1 and TGF-beta 1 antibody were used to treat EOC cells and analyzed whether TGF-beta 1 contributed to the cell cycle arrest. Results from in vivo and ex vivo experiments showed that hAEC-secreted factors and rhTGF-beta 1 decreased proliferation of EOC cells and induced G0/G1 cell cycle arrest in cancer cells, which could be partially reversed by excess TGF-beta 1 antibody. These data indicate that hAECs endow potential anticancer properties on epithelial ovarian cancer in vivo and in vitro which is partially mediated by hAEC-secreted TGF-beta 1-induced cell cycle arrest. This study suggests a potential application of hAEC-based therapy against epithelial ovarian cancer.
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