期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 50, 期 4, 页码 1251-1260出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2017.3888
关键词
Snail; epithelial-to-mesenchymal transition; non-small cell lung cancer; cell cycle
类别
资金
- National Key Research and Development Program of China [TFA 0500303]
- National Natural Science Foundation of China [8133005, 81372594, 81201964]
- '863' Project [2014AA021606, 2015AA020403]
- Beijing Natural Science Foundation [5122012, 7132051]
Epithelial-to-mesenchymal transition (EMT) is essential for tumor invasion and metastasis. Snail has been proven to be a key regulator of EMT. Several studies have shown compelling evidence that Snail is also an important regulator of tumor growth and aggression; however, the role of Snail in the cell cycle has not been clarified. We decreased Snail expression by siRNA transfection and lentiviral-mediated RNAi, to explore the effect of silencing Snail on the tumorigenicity and migration of lung carcinoma (lung cancer) cells. The results showed that silencing Snail conferred significant anti-proliferative activity and inhibited cell migration, tumor growth and metastasis both in vitro and in vivo. To understand the mechanism of these effects, we further investigated correlations among Snail expression, EMT and cell cycle. Significantly, Snail knockdown reversed EMT processes in lung cancer cells. Furthermore, the cyclin-dependent kinase inhibitor P21 was upregulated after silencing Snail. P21 upregulation manifested its tumor suppressor effects and arrested cells in the G2/M phase, not the G1/S phase following Snail depletion in lung cancer cells. These data suggest that silencing Snail decreases the malignant behaviors of lung cancer cells by reversing EMT processes and causing cell cycle defects.
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