期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 12, 期 -, 页码 3049-3058出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S131805
关键词
polythionine-Au composites; label-free electrochemical immunoassay; carbohydrate antigen 19-9; clinical sample; signal amplification
资金
- National Natural Science Foundation of China [21275028, 21405016]
- Natural Science Foundation of Fujian Province [2015J01595, 2015J01043, 2017J01532]
- Medical Elite Cultivation Program of Fujian, People's Republic of China [2014-ZQN-JC-23]
- Scientific Research Major Program of Fujian Medical University [JS15004]
- Nursery Research Fund of Fujian Medical University [2014MP034]
- Education and Scientific Research Project of Young and Middle-aged Teachers in Fujian Province of China [JAT160198]
- Sailing Research Fund of Fujian Medical University [2016QH013]
- Opening Foundation of State Key Laboratory of Physical Chemistry of Solid Surfaces (Xiamen University)
Carbohydrate antigen 19-9 (CA19-9) is an important biomarker for the early diagnosis and clinical monitoring of pancreatic cancer. Reliable, simple, and accurate methods for the detection of CA19-9 are still urgently needed. In this study, polythionine-Au composites (AuNPs@PThi) were designed and prepared through one-pot reaction using HAuCl4 as the co-oxidant and raw material in thionine solution containing FeCl3 as the oxidant. AuNPs@PThi-immobilized glassy carbon electrode was used as a sensitive redox probe for electrochemical interface. AuNPs@PThi not only favored the amplification of electrochemical signals but also facilitated excellent environmental friendliness for bioassay. Maximizing the electrochemical properties of AuNPs@PThi, an effective label-free electrochemical immunoassay for the ultrasensitive and reliable detection of CA19-9 was developed. Under optimal conditions, the linear range of the proposed immunosensor was estimated to range from 6.5 to 520 U/mL, with a detection limit of 0.26 U/mL at a signal-to-noise ratio of 3. The prepared immunosensor for CA19-9 detection showed high sensitivity, stability, and reproducibility. Furthermore, the fabricated immunosensor based on AuNPs@PThi can effectively detect and distinguish clinical serum samples of pancreatic cancer and normal control with accuracy and convenience.
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