期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 18, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/ijms18112290
关键词
fibrosis; TGF beta; ischemia; cardiac remodeling; endothelial cells; cardiomyocytes; apoptosis
资金
- Deutsche Forschungsgemeinschaft [EU 121/2-3]
- Thyssen Stiftung [Az. 10.11.2.142]
Cardiac remodeling plays a crucial role in the development of heart failure after mycocardial infarction. Besides cardiomyocytes, endothelial cells are recognized to contribute to cardiac remodeling. We now investigated processes of endothelial mesenchymal transition (EndoMT) in microvascular endothelial cells of rat (MVEC) under hypoxia and paracrine effects on ventricular cardiomyocytes of adult rat. Exposure of MVECs to hypoxia/reoxygenation enhanced TGF beta/SMAD signaling, since phosphorylation, and thus activation, of SMAD1/5 and SMAD2 increased. This increase was blocked by inhibitors of TGF beta receptor types ALK1 or ALK5. Exposure of ventricular cardiomyocytes to conditioned medium from hypoxic/reoxygenated MVECs enhanced SMAD2 phosphorylation and provoked apoptosis in cardiomyoyctes. Both were blocked by ALK5 inhibition. To analyze autocrine effects of hypoxic TGF beta signaling we investigated EndoMT in MVECs. After 3 days of hypoxia the mesenchymal marker protein alpha-smooth muscle actin (alpha-SMA), and the number of alpha-SMA- and fibroblast specific protein 1 (FSP1)-positive cells increased in MVECs cultures. This was blocked by ALK5 inhibition. Similarly, TGF beta(1) provoked enhanced expression of alpha-SMA and FSP1 in MVECs. In conclusion, hypoxia provokes EndoMT in MVECs via TGF beta(1)/SMAD2 signaling. Furthermore, release of TGF beta(1) from MVECs acts in a paracrine loop on cardiomyocytes and provokes apoptotic death. Thus, in myocardial infarction hypoxic endothelial cells may contribute to cardiac remodeling and heart failure progression by promotion of cardiac fibrosis and cardiomyocytes death.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据