期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 18, 期 4, 页码 -出版社
MDPI AG
DOI: 10.3390/ijms18040683
关键词
uterine contractility; tissue bio-printing; contractility assay; myometrium; patient-derived; tocolytics; personalization of therapy
资金
- Small Business Innovation Research (SBIR) Phase I award from the Eunice Kennedy Shriver National Institute of Childhood Health and Human Development (NICHD) of the National Institutes of Health (NIH) [R41HD081795]
- Center for the Advancement of Science in Space (CASIS)
- NICHD/NIH [R21 HD082947-01A1]
Deregulation in uterine contractility can cause common pathological disorders of the female reproductive system, including preterm labor, infertility, inappropriate implantation, and irregular menstrual cycle. A better understanding of human myometrium contractility is essential to designing and testing interventions for these important clinical problems. Robust studies on the physiology of human uterine contractions require in vitro models, utilizing a human source. Importantly, uterine contractility is a three-dimensionally (3D)-coordinated phenomenon and should be studied in a 3D environment. Here, we propose and assess for the first time a 3D in vitro model for the evaluation of human uterine contractility. Magnetic 3D bioprinting is applied to pattern human myometrium cells into rings, which are then monitored for contractility over time and as a function of various clinically relevant agents. Commercially available and patient-derived myometrium cells were magnetically bioprinted into rings in 384-well formats for throughput uterine contractility analysis. The bioprinted uterine rings from various cell origins and patients show different patterns of contractility and respond differently to clinically relevant uterine contractility inhibitors, indomethacin the physiology of human parturition while enabling high-throughput testing of multiple agents and conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据