Resveratrol inhibits TNF-α-induced inflammation to protect against renal ischemia/reperfusion injury in diabetic rats

Purpose: To examine effects of resveratrol on renal ischemia/ reperfusion injury (I/R) in a streptozotocin (STZ)-induced diabetic rat model. Methods: Twenty-four male Sprague Dawley rats were treated with STZ injection for the development of diabetes, and divided into the following groups: Sham group, I/R group and Resveratrol group (n=8). Resveratrol (RSV) was administered at a dose of 10 mg.kg(-1).d(-1) fourteen days prior to suffering from I/R. Renal function, histology, SOD, MDA, TUNEL assay and expression of TNF-alpha, IL-1 beta, NF-kappa B-P65, COX-2 and Caspase3, Bcl2 and Bax were analyzed. Results: Administration of RSV significantly reduced the serum levels of renal dysfunction and injury markers, including-creatinine, blood urea nitrogen and MDA; in the other hand, it significantly increased the serum levels of SOD. The protective effect of RSV was also reflected on histologic evaluation. RSV reduced the number of a poptotic cells as determined by TUNEL assay. RSV significantly reduced the protein expression of TNF-alpha, IL-1 beta, NF-kappa B-P65, COX-2 and Caspase3, and Bax. Meanwhile, RSV significantly increased the protein expression of Bcl2. Conclusion: RSV attenuated I/R-induced renal injury in diabetic rats through the modulation of oxidative stress and TNF-alpha-stimulated inflammation.