期刊
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
卷 40, 期 5, 页码 1529-1536出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2017.3138
关键词
hypoxia; hypoxia-inducible factor-1 alpha; HIF1A antisense RNA 1; HIF1A antisense RNA 2; osteogenic differentiation; periodontal ligament cells
资金
- Natural Science Foundation of Guangdong Province [2015A030313083]
- Natural Science Foundation of China [81400499]
Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is essential for regulating the osteogenic differentiation of periodontal ligament cells (PDLCs). The regulatory mechanism of HIF-1 alpha transcription is still not clear. Recently, two long non-coding RNAs, HIF1A antisense RNA 1 (HIF1A-AS1) and HIF1A antisense RNA 2 (HIF1A-AS2), were found to regulate HIF-1 alpha mRNA, but the regulatory mechanisms among HIF-1 alpha, HIF1A-AS1 and HIF1A-AS2 have not been well studied. We hypothesized that HIF1A-AS1 and HIF1A-AS2 play important roles in the osteogenic differentiation of PDLCs by regulating HIF-1 alpha. In the present study, we showed that expression levels of HIF1A-AS1, HIF1A-AS2, HIF-1 alpha and osteogenic biomarkers were time-dependent under hypoxia. Even though both HIF1A-AS1 and HIF1A-AS2 were complementary to HIF-1 alpha mRNA, only HIF1A-AS2 showed an inhibitory effect on HIF-1 alpha in PDLCs. Moreover, HIF-1 alpha had positive regulatory effects on HIF1A-AS1 and HIF1A-AS2. HIF-1 alpha promoted the osteogenic differentiation of PDLCs, and HIF1A-AS2 had a negative effect on the osteogenic differentiation of PDLCs. Altogether, the present study revealed the complex relationships among HIF1A-AS1, HIF1A-AS2 and HIF-1 alpha, as well as their roles in regulating the osteogenic differentiation of PDLCs. These findings provide a theoretical basis for promoting periodontal tissue regeneration and repair during orthodontic tooth movement.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据