期刊
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
卷 19, 期 1, 页码 65-73出版社
BEGELL HOUSE INC
DOI: 10.1615/IntJMedMushrooms.v19.i1.70
关键词
ganoderic acid; Ganoderma lucidum; gene expression; medicinal mushrooms; salicylic acid
资金
- National Natural Science Foundation of China [31400035, 31301825]
- Chinese Postdoctoral Science Foundation [2015M570457]
- Fundamental Research Funds for the Central Universities [KJQN201527]
- Jiangsu Planned Projects for Postdoctoral Research Funds [1402020B]
- Key Research and Development Program of Jiangsu Province [BE2014338, BE2015361]
We demonstrate herein that salicylic acid (SA) can enhance ganoderic acid (GA) accumulation in the lingzhi or reishi medicinal mushroom Ganoderma lucidum. Following treatment with different concentrations of SA, the GA content was increased 22.72% to 43.04% compared with the control group. When the fungi were treated with 200 mu mol/L SA at different times, the GA content was improved 10.21% to 35.24% compared with the control group. By choosing the optimum point based on response surface methodology, the GA content could be increased up to 229.03 mu g/100 mg, which was improved 66.38% compared with the control group. When the fungi were treated with 200 mu mol/L SA, the transcription levels of key genes in the GA biosynthesis pathway-squalene (SQ) synthase (sqs), lanosterol (Lano; osc), and hydroxy-3-methylglutaryl-coenzyme A reductase (hmgr)-were improved 119.6-, 3.2-, and 4.2-fold, respectively. In addition, following treatment with 100 mu mol/L SA, the levels of Lano and SQ, which are intermediate metabolites of GA biosynthesis, were increased 2.8-and 1.4-fold, respectively. These results indicate that SA can regulate the expression of genes related to GA biosynthesis and increases the metabolic levels of Lano and SQ, thereby resulting in the accumulation of GA.
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