期刊
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
卷 19, 期 11, 页码 1029-1040出版社
BEGELL HOUSE INC
DOI: 10.1615/IntJMedMushrooms.2017024487
关键词
Ganoderma lucidum; heat stress; medicinal mushrooms; reference genes; RT-qPCR
资金
- National Natural Science Foundation of China [31400035, 31672212]
- Fundamental Research Funds for the Central Universities [KJQN201527]
- Key Research and Development Program of Jiangsu Province [BE2015361]
Ganoderma lucidum has been considered an emerging model species for studying how environmental factors regulate the growth, development, and secondary metabolism of Basidiomycetes. Heat stress, which is one of the most important environmental abiotic stresses, seriously affects the growth, development, and yield of microorganisms. Understanding the response to heat stress has gradually become a hotspot in microorganism research. But suitable reference genes for expression analysis under heat stress have not been reported in G. lucidum. In this study, we systematically identified 11 candidate reference genes that were measured using reverse transcriptase quantitative polymerase chain reaction, and the gene expression stability was analyzed under heat stress conditions using geNorm and NormFinder. The results show that 5 reference genes-CYP and TIF, followed by UCE2, ACTIN, and UBQ1-are the most stable genes under our experimental conditions. Moreover, the relative expression levels of 3 heat stress response genes (hsp17.4, hsp70, and hsp90) were analyzed under heat stress conditions with different normalization strategies. The results show that use of a gene with unstable expression (SAND) as the reference gene leads to biased data and misinterpretations of the target gene expression level under heat stress.
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