期刊
BIO-DESIGN AND MANUFACTURING
卷 3, 期 4, 页码 361-372出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s42242-020-00085-5
关键词
Biomaterial; Structure-activity relationship; Hydroxyethyl cellulose
资金
- National Natural Science Foundation of China [21675017]
- State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products [KF20190108]
- National Key Research and Development Program of China [2017YFC1702001]
In this paper, we present a 3D printed tumor spheroidal model suitable for drug discovery. This model is based on a hydroxyethyl cellulose/alginate/gelatin (HCSG) composite biomaterial that has three distinct properties: (1) the HCSG is similar to the commercial basement membrane extract in Ki67, MUC1, and PARP1 expressions of MCF-7 cells for embedding culture; (2) the HCSG is printable at room temperature; and (3) the HCSG can be large-scale manufactured at an ultralow cost. We printed a 3D MCF-7 spheroid model with HCSG and characterized it in terms of cell viability, spheroid size, key protein expression, and mitochondrial metabolic activity. We used the 3D MCF-7 spheroid model to evaluate the anti-breast cancer activity of 13 amino acid-based flavone phosphoramidates and found that the alanine structure induced a stronger drug resistance, whereas phenylalanine hardly caused drug resistance in the MCF-7 cells. This is the first time that 3D bioprinting technology has been used in a structure-activity relationship study.
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