期刊
EMERGING MICROBES & INFECTIONS
卷 9, 期 1, 页码 1080-1091出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/22221751.2020.1760735
关键词
Vaccine; MERS-coronavirus; spike; i301; lumazine synthase; spytag-spycatcher; rabbit; SARS-CoV-2
资金
- IMI-ZAPI (Zoonotic Anticipation and Preparedness Initiative (ZAPI) project
- Innovative Medicines Initiative (IMI)) [115760]
- IMI
- European Commission
Middle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein -fusion peptide, heptad repeat 2, and receptor binding domain (RBD) - and tested their immunogenicity and protective capacity in rabbits. Using a plug-and-display SpyTag/SpyCatcher system, we coupled RBD to lumazine synthase (LS) particles producing multimeric RBD-presenting particles (RBD-LS). RBD-LS vaccination induced antibody responses of high magnitude and quality (avidity, MERS-CoV neutralizing capacity, and mucosal immunity) with cross-clade neutralization. The antibody responses were associated with blocking viral replication and upper and lower respiratory tract protection against MERS-CoV infection in rabbits. This arrayed multivalent presentation of the viral RBD using the antigen-SpyTag/LS-SpyCatcher is a promising MERS-CoV vaccine candidate and this platform may be applied for the rapid development of vaccines against other emerging viruses such as SARS-CoV-2.
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