期刊
NANOSCALE
卷 12, 期 28, 页码 15050-15053出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0nr02961k
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资金
- Ministerio de Ciencia, Innovacion y Universidades [PCI2018-093064, PGC2018-094802-B-I00, SAF2017-87305-R, SEV-2016-0686]
- Comunidad Autonoma de Madrid [S2017/BMD-3867]
We report the use of the amyloid probe Thioflavin T (ThT) as a specific and exchangeable fluorophore for stimulated emission depletion (STED) super-resolution imaging of amyloid fibers. This method achieves a spatial resolution in the range of 60-70 nm, low image background and increased photostability that enables long-term STED imaging. These results expand the widespread uses of ThT and can be potentially extended to other common amyloid fluorescent probes, providing new tools for the study of amyloid diseases.
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