期刊
ANALYST
卷 145, 期 14, 页码 4777-4781出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0an00723d
关键词
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资金
- National Research Foundation of Korea (NRF) [2017R1A2B4002398]
- National University Development Project in 2019
- National Research Foundation of Korea [2017R1A2B4002398] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study we synthesized the nucleotidedUrkTP, a highly fluorescent naphthalimide deoxyuridine triphosphate that undergoes aggregation-induced emission (AIE). We incorporated and extendeddUrkTPduring the primer extension of DNA mediated by DNA polymerase, and also in the rolling circle amplification of DNA mediated by Phi29 polymerase. Accordingly, we could use this fluorescent nucleotide for the detection of microRNA 24-3P, a biomarker of porcine reproductive and respiratory syndrome virus. The direct labeling system obtained during rolling circle DNA amplification exhibited increased fluorescence, due to AIE of thedUrkTPresidue upon gel formation, thereby allowing the detection of miRNA 24-3P. This direct labeling system facilitated the simple and inexpensive detection of miRNA 24-3P with high sensitivity (limit of detection: 3.58 fM) and selectivity.
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