期刊
ADVANCED BIOSYSTEMS
卷 4, 期 9, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adbi.202000004
关键词
3D culture; glaucoma; microfabrication; outflow; stem cell; trabecular meshwork
资金
- SUNY Poly Seed Grant Program
- New York State Center for Advanced Technology in Nanomaterials and Nanoelectronics (CATN2) Matching Investment Program (MIP)
- John J. Sullivan Award
- National Institute of Health [R01 EY025543, R01 EY025643]
- National Science Foundation Small Business Innovation Research Program Phase II [1660131]
- Directorate For Engineering [1660131] Funding Source: National Science Foundation
- Div Of Industrial Innovation & Partnersh [1660131] Funding Source: National Science Foundation
Age-related human trabecular meshwork (HTM) cell loss is suggested to affect its ability to regulate aqueous humor outflow in the eye. In addition, disease-related HTM cell loss is suggested to lead to elevated intraocular pressure in glaucoma. Induced pluripotent stem cell (iPSC)-derived trabecular meshwork (TM) cells are promising autologous cell sources that can be used to restore the declining TM cell population and function. Previously, an in vitro HTM model is bioengineered for understanding HTM cell biology and screening of pharmacological or biological agents that affect trabecular outflow facility. In this study, it is demonstrated that human iPSC-derived TM cells cultured on SU-8 scaffolds exhibit HTM-like cell morphology, extracellular matrix deposition, and drug responsiveness to dexamethasone treatment. These findings suggest that iPSC-derived TM cells behave like primary HTM cells and can thus serve as reproducible and scalable cell sources when using this in vitro system for glaucoma drug screening and further understanding of outflow pathway physiology, leading to personalized medicine.
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