期刊
FOOD & FUNCTION
卷 11, 期 7, 页码 6595-6607出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0fo01193b
关键词
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资金
- China Scholarship Council (CSC)
- National Natural Science Foundation of China [31171768]
- program for Guangdong Introducing Innovative and Entrepreneurial Teams [2019ZT08N291]
- Ministry of Science and Technology of China [2016YFD0600806]
- Clinical Chinese Medicine Plateau Discipline Construction Project of Shanghai Pudong New Area Health Committee [PDZY-20180604]
We extracted and purified oxyresveratrol (OXY) fromArtocarpus heterophyllusLam. and identified its structure. The kinetics and mechanisms of OXY-induced mushroom tyrosinase inhibition were studied using fluorescence spectroscopy, copper ion chelation, and circular dichroism (CD). We found that OXY significantly inhibited tyrosinase with a half maximal inhibitory concentration (IC50) of 0.03 mM. The inhibitory effect of OXY on tyrosinase was almost 25 times that of kojic acid, which had an IC(50)of 0.78 mM. Additionally, OXY and the tyrosinase substrate L-dopa did not have a competitive relationship; OXY is a non-competitive inhibitor. Using a fluorescence quenching experiment, we determined the corresponding rate constant (K-q) values at 298, 303, and 310 K to be 2.24 x 10(12), 1.08 x 10(12)and 1.44 x 10(12)L mol(-1)s(-1), respectively. The OXY and tyrosinase interaction occured mainly through van der Waals forces and a hydrogen bond between the -OH group and its amino acid residue. Furthermore, we investigated the effects of OXY on murine melanoma B16 cells and on age pigments inCaenorhabditis elegans(C. elegans). OXY decreased melanin production by inhibiting the tyrosinase activity in murine melanoma B16 cells, which decreased superoxide dismutase (SOD) and glutathione peroxidase (GSH) and increased catalase (CAT), leading to apoptosis. The lifespan of nematodes in the 50 ml resveratrol-treated group was significantly longer than that in the blank group by 5%. The mean lifespan of nematodes in the 50 mu M OXY-treated group was significantly longer than that in the blank group by 6.82%.The fluorescence intensity ofC. eleganspigments decreased by 30.43%, 47.35% and 64.42% after the treatment with a low, middle, and high OXY dose, respectively, showing that OXY has a significant inhibitory effect on melanin and age pigment production.
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