期刊
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
卷 18, 期 1, 页码 -出版社
SPRINGERNATURE
DOI: 10.1186/s43141-020-00043-9
关键词
Metagenomics; Novel enzymes; Microbial community; Environmental DNA
资金
- Faculty of Science and Mathematics-Diponegoro University - Indonesia under the scheme of PNBP
Background Microbial community has an essential role in various fields, especially the industrial sector. Microbes produce metabolites in the form of enzymes, which are one of the essential compounds for industrial processes. Unfortunately, there are still numerous microbes that cannot be identified and cultivated because of the limitations of the culture-based method. The metagenomic approach is a solution for researchers to overcome these problems. The main body of the abstract Metagenomics is a strategy used to analyze the genomes of microbial communities in the environment directly. Metagenomics application used to explore novel enzymes is essential because it allows researchers to obtain data on microbial diversity, reaching of 99% and various types of genes encoding an enzyme that has not yet been identified. Basic methods in metagenomics have been developed and are commonly used in various studies. A basic understanding of metagenomics for researchers is needed, especially young researchers to support the success of the research. Short conclusion Therefore, this review was done in order to provide a deep understanding of metagenomics. It also discussed the application and basic methods of metagenomics in the exploration of novel enzymes, especially in the latest research. Several types of enzymes, such as cellulases, proteases, and lipases, which have been explored using metagenomics, were reviewed in this article.
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