期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
卷 69, 期 5, 页码 584-597出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09637486.2017.1401980
关键词
Epigallocatechin-3-O-gallate; mitochondrial membrane potential; ROS; cancer; signalling pathways
资金
- National Research, Development and Innovation Office, Hungary [K103906, K120302, GINOP-2.3.2-15-2016-00020, GINOP-2.3.2-15-2016-00050]
In this study, we evaluated the effects of epigallocatechin-3-O-gallate (EGCG) in two cancer cell lines, A-431 overexpressing ErbB1 and SK-BR-3, overexpressing ErbB2. EGCG treatment showed dose-dependent collapse of mitochondrial membrane potential ((m)), increase in reactive oxygen species (ROS) production, changes in nuclear morphology and reduced viability. Flow cytometry data indicated that EGCG partially decreases the phosphorylation of several proteins involved in cell proliferation and survival: pErbB1(Y1173, Y1068), pAkt(S473) and pERK(Y204). EGCG affected the clonogenic growth in both cell lines with an EC50 of 2.5 and 5.4 mu M for A-431 and SK-BR-3, respectively. Wound scratch assay demonstrated that EGCG inhibited the healing in dose-dependent manner and the effect was correlated with partial reduction in phosphorylation of pFAK(S910). Our data suggest that EGCG administration might reduce the unfavourable traits, particularly associated with ErbB1/EGFR overexpression.
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