期刊
NATURAL PRODUCT REPORTS
卷 37, 期 7, 页码 893-918出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9np00068b
关键词
-
资金
- National Cancer Institute, National Institutes of Health [HHSN261200800001E]
- National Cancer Institute
- Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research
- NCI Division of Cancer Treatment and Diagnosis Developmental Therapeutics Program
- NATIONAL CANCER INSTITUTE [ZIABC011904, ZIABC011471, ZIABC011854] Funding Source: NIH RePORTER
Covering: up to 2020 The National Cancer Institute of the United States (NCI) has initiated a Cancer Moonshot program entitled the NCI Program for Natural Product Discovery. As part of this effort, the NCI is producing a library of 1 000 000 partially purified natural product fractions which are being plated into 384-well plates and provided to the research community free of charge. As the first 326 000 of these fractions have now been made available, this review seeks to describe the general methods used to collect organisms, extract those organisms, and create a prefractionated library. Importantly, this review also details both cell-based and cell-free bioassay methods and the adaptations necessary to those methods to productively screen natural product libraries. Finally, this review briefly describes post-screen dereplication and compound purification and scale up procedures which can efficiently identify active compounds and produce sufficient quantities of natural products for further pre-clinical development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据