期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 27, 期 -, 页码 64-74出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2015.06.002
关键词
-
资金
- MOB program of the Russian Academy of Sciences
- Russian Foundation for Basic Research Grant [14-03-31162 mol_a, 13-04-01878a]
- National Science Foundation [CHE-1213047, CHE-1425951]
- National Institute of Health [R21EB009976-01]
- Direct For Mathematical & Physical Scien [1213047] Funding Source: National Science Foundation
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB009976] Funding Source: NIH RePORTER
Green fluorescent protein and related proteins carry chromophores formed within the protein from their own amino acids. Corresponding synthetic compounds are nonfluorescent in solution due to photoinduced isomerization of the benzylideneimidiazolidinone core. Restriction of this internal rotation by binding to host molecules leads to pronounced, up to three orders of magnitude, increase of fluorescence intensity. This property allows using GFP chromophore analogs as fluorogenic dyes to detect metal ions, proteins, nucleic acids, and other hosts. For example, RNA aptamer named Spinach, which binds to and activates fluorescence of some GFP chromophores, was proved to be a unique label for live-cell imaging of specific RNAs, endogenous metabolites and target proteins. Chemically locked GFP chromophores are brightly fluorescent and represent potentially useful dyes due to their small size and high water solubility.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据