期刊
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
卷 20, 期 -, 页码 S430-S443出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2017.1297953
关键词
Canned product; DNA isolation; Fish-based food; PCR; Thunnus albacares
资金
- Ministry of Agriculture of the Czech Republic [QJ1530107]
- Internal Grant Agency of the University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic [229/2016/FVHE]
The most common techniques used to identify tuna species include methods based on the detection of specific DNA. The quality of species-specific DNA crucially affects the efficiency of amplification during the subsequent polymerase chain reaction (PCR). Detection of DNA in processed products can be adversely affected by DNA fragmentation during the processing steps and the use of ingredients that may inhibit the PCR reaction. In this study, several processing treatments applied to the muscle tissue of yellowfin tuna (Thunnus albacares) were evaluated. For DNA isolation three DNA extraction methods were compared. The concentration, purity, and amplificability of DNA were tested. The results revealed the variability among extraction procedures in terms of DNA quality and quantity in tuna muscle tissue processed under different processing technologies.
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