期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 8, 期 16, 页码 3513-3518出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9tb02478f
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资金
- National Natural Science Foundation of China [21575071, 21874079]
- Nature Science Foundation for Outstanding Young Scientists of Shandong Province [ZR2018JL011]
- Science & Technology Fund Planning Project of Shandong Colleges and Universities [J16LA13, J18KA112]
- Qingdao Science & Technology Planning Project [17-6-3-15-gx]
Nanozyme-based colorimetry was suggested to be a rapid method for biomarker (e.g. glutathione) detection, but this method suffers from lack of efficiency and low-toxicity nanozymes till now. Herein, quantum dots of TiO2 loaded on carbon (TiO2/C-ODs) oxidase-like nanozymes were prepared via a hydrothermal treatment of tiny and few-layered Ti3C2Tx MXene nanosheets, which possess abundant thermodynamic metastable Ti atoms on MXene margins as raw materials for the preparation of TiO2/C-ODs. The oxygen vacancy in TiO2 on the surface of the carbon matrix can facilitate O-2 adsorption in the solution and generate reactive oxygen species (ROS), thereby quickly oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) to its oxidized form (TMBox) in the absence of H2O2. After adding glutathione (GSH), TMBox was able to be restored to TMB, which resulted in a corresponding decrease in the UV-vis absorbance value at 652 nm. Furthermore, this assay possesses good selectivity, excellent specificity and high sensitivity (limit of detection: 0.2 mu M), which made it possible to efficiently detect GSH in complex biological samples such as human serum.
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