期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 31, 期 1, 页码 587-599出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2020.1749570
关键词
Zeolitic imidazolate frameworks; carboxypeptidase A; immobilization; degradation; ochratoxin A
资金
- Natural Science Foundation of Jiangsu Province, China [BK20180979]
- Natural Science Research of Jiangsu Higher Education Institutions of China [18KJB550003, 19KJB550006]
- Doctoral Research start-up Funds of Jiangsu University Of Science And Technology [1182931801]
- Emerging science and technology innovation team funding of JUST [1182921902]
- Zhenjiang Scientific and Technological Foundation for Social Development [SH2017053]
- National Natural Science Foundation of China [31901799]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [SJCX19_0606]
Ochratoxin A (OTA) is a highly toxic potential contaminant of most agricultural products, and OTA is difficult to remove in general food production processes. In this study, we immobilized polyvinyl pyrrolidone-coated carboxypeptidase A (CPA) into zeolitic imidazolate framework material to improve the ability for OTA degradation. In this method, carboxypeptidase A was dispersed well in the material and maintained its stability and ability to degrade OTA, under complex matrix conditions with better repeated use performance than free carboxypeptidase A. The substrate conversion rate of CPA increased 3 times after immobilization. After repeated use of 10 cycles, the composite enzyme still retained 71.37% of its initial activity. The degradation rate of OTA was 30.69% higher than that of free CPA. This method may play an important role in the degradation of mycotoxins.
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