Electrochemical Detection of Vitamin D2 and D3 Based on a Au-Pd Modified Glassy Carbon Electrode

In this work, the synthesis of AuPd bimetallic nanocrystals in an aqueous solution using a stabilizing agent and a reductant of triblock copolymer P123 and ascorbic acid, respectively, was proposed. Vitamins D-2 and D-3 were electrochemically detected in a mixed organic/water solution based on a glassy carbon electrode (GCE) modified by AuPd. The electrocatalytic response of D vitamins on the GCE surface was greatly affected by the organic/water ratio of the mixture. In the presence of the support electrolyte (lithium perchlorate), vitamins D-2 and D-3 exhibited well-defined peaks when the ethanol/water ratio was 40%/60%. This work also suggested the high sensitivity of the GCE toward the detection of vitamins D-2 and D-3. During the detection of vitamin D, vitamins A, K and E showed no obvious interference effects.