Thioglycoligation of aromatic thiols using a natural glucuronide donor

The beta-d-glucuronidaseDtGlcA fromDictyoglomus thermophilumwas engineered to generate an active thioglycoligase that is able to catalyse the formation of numerousS-glucuronides. Its X-ray structure analysis indicated the ability of the biocatalyst to bind aromatic thiol acceptors forS-glycosylation. Noteworthily, theDtGlcA mutant was found to be the first thioligase that is able to use a natural sugar donor different from the widely used syntheticpara-nitrophenyl glycosides.