Single nucleotide variant discrimination by toehold exchange spherical nucleic acids modulated on hierarchical molybdenum disulfide acanthospheres

Toehold exchange spherical nucleic acids (TESNA) modulated on molybdenum disulfide (MoS2) acanthospheres were proposed for the discrimination of single nucleotide variants (SNVs). We rationally optimized the probe by making a trade-off between the discrimination factor (DF) and hybridization ratio (v). The TESNA platform can distinguish SNVs with significantly improved sensitivity and specificity.