期刊
BIOSCIENCE OF MICROBIOTA FOOD AND HEALTH
卷 39, 期 3, 页码 89-99出版社
BMFH PRESS
DOI: 10.12938/bmfh.2019-025
关键词
heat-inactivated lactobacilli; antibiotics; gut microbiota
资金
- Public Health and Preventive Medicine Provincial Experiment Teaching Center at Sichuan University
- Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province
This study was conducted to evaluate the possibility of using heated-inactivated lactobacilli to protect neonates from harmful effects of antibiotics. Thirty neonate mice were randomly divided into three groups of ten and treated with either sterilized water, an antibiotics cocktail, or the same antibiotics plus heat-inactivated Lactobacillus paracasei N1115. The administration of antibiotics significantly increased the serum interleukin-6 (IL-6) levels of the tested mice (p<0.01, p<0.001, respectively) and decreased their serum corticosterone levels (p<0.01, p<0.01, respectively). The colonic crypts were significantly less deep in mice treated with antibiotics and with antibiotics plus N1115 (p<0.05). Antibiotics caused significantly abnormal expression of brain-derived neurotrophic factor (BDNF), gamma-aminobutyric acid type A receptor alpha 1 (GABA(A alpha 1)), gamma-aminobutyric acid type B receptor1 (GABA(b1)), and 5-hydroxytryptamine receptor1A (5-HT1A) in the hippocampus (p<0.05, p<0.01, p<0.01, respectively) and of GABA(A alpha 1) in the prefrontal cortex (p<0.01). Heat-inactivated lactobacilli alleviated these abnormal changes. Antibiotics greatly decreased the Shannon index of the fecal microbiota and significantly increased the number of Proteobacteria (p<0.001), with fewer Bacteroidetes and Firmicutes (p<0.05). Antibiotics not only cause microbiota dysbiosis, but also cause abnormal changes in important molecules in the gut-brain axis. All these abnormal changes are alleviated by heat-inactivated L. paracasei N1115. This indicates that heat-inactivated L. paracasei N1115 has a certain improvement effect on changes caused by antibiotics.
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