Anti-inflammatory and anticancer activities of water-soluble peptide extracts of buffalo and cow milk Cheddar cheeses

This article aimed to assess the anti-inflammatory and anticancer potential of water-soluble peptide (WSP) extracts from buffalo and cow milk Cheddar cheeses. Anti-inflammatory activity was evaluated on the basis of nitric oxide (NO) production in lipopolysaccharide-stimulated macrophage (RAW-264.7) cells. A cell viability assay, cell cycle arrest and apoptosis were performed to explore anticancer activity in a colon cancer model (HT-29). The WSP extracts of both Cheddar cheeses effectively inhibited NO production in activated macrophages. Maximum growth inhibition was observed in the HT-29 cells at concentrations of 400 and 500g/mL. A significant increase in cell population at G(0)/G(1) phase of the cell cycle was observed. Moreover, the WSP extracts also induced extensive apoptosis in colon cancer cells.