期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 8, 期 30, 页码 6469-6480出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9tb02648g
关键词
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资金
- National Natural Science Foundation of China (NNSFC) [51573072]
- MOE AMP
- SAFEA for the 111 Project [B13025]
Specific recognition and sensing of glycoproteins are of great importance in clinical diagnostics considering their frequent utilization as biomarkers and therapeutic targets. In this work, a biomimetic fluorescent sensor for the selective and sensitive detection of glycoprotein was developed, which was based on late-model boronate fluorescent molecularly imprinted nanoparticles (B-FMIP NPs). The B-FMIP NPs were fabricatedviathe macromolecular assembly of a fluorescent photo-crosslinkable amphiphilic copolymer containing boronic acid with glycoprotein in aqueous solution andin situphoto-crosslinking. Due to the synergism of boronate affinity and the molecular imprinting effect, the resultant B-FMIP NPs demonstrated specific recognition and remarkable selectivity toward the template glycoprotein (ovalbumin, OVA) with a high imprinted factor (alpha) of 6.0 and gave rise to obvious fluorescence quenching after binding with OVA in water. Under optimized experimental conditions, the as-prepared B-FMIP NPs exhibited linearity over the OVA concentration range of 10(-13)to 10(-3)mg mL(-1)with a detection limit of 3.3 x 10(-14)mg mL(-1), as well as a rapid response time (about 10 min), which was superior to that of other previously reported OVA sensors. Finally, these B-FMIP NPs have been applied for the determination of OVA in real samples.
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