期刊
SENSING AND BIO-SENSING RESEARCH
卷 28, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.sbsr.2020.100333
关键词
3-nitrotyrosine; Molecular imprinted polymer; Electrochemical (bio)sensor; Urine biomarker; Carbon-printed electrode
资金
- Fundacao para a Ciencia e Tecnologia (FCT) [PTDC/AAG-TEC/5400/2014, POCI-01-0145-FEDER-016637, NORTE-01-0145-FEDER-024358, UID/CTM/50025/2019, SFRH/BD/94159/2013, SFRH/BD/115173/2016]
- H2020 [MindGAP/FET-Open/GA829040]
- Fundação para a Ciência e a Tecnologia [PTDC/AAG-TEC/5400/2014, SFRH/BD/115173/2016, SFRH/BD/94159/2013] Funding Source: FCT
Over the last years, paper technology has been widely spread as a more affordable, sustainable and reliable support material to be incorporated in the design of point-of-care (POC) diagnostic devices. However, the single work employing a paper-based device for 3-nitrotyrosine (3-NT), a relevant biomarker for oxidative stress (OS) that is a major origin for many diseases, is incapable of reading successfully complex samples because every species that oxidizes before similar to 0.75 V will also contribute to the final response. Thus, the introduction of a selective element was made into this set-up by including a molecularly-imprinted polymer (MIP) tailored in-situ. Herein, a novel MIP for 3-NT was assembled directly on a paper platform, made conductive with carbon ink and suitable for an electrochemical transduction. The biomimetic material was produced by electropolymerization of phenol after optimizing several experimental parameters, such a scan-rate, number of cycles, range of potential applied, monomer and template concentrations. Under optimal conditions, the label-free sensor was able to respond to 3-NT from 500 nM to 1 mM, yielding a limit of detection of 22.3 nM. Finally, the applicability of the (bio)sensor was tested by performing calibration assays in human urine samples and a good performance was obtained in terms of sensitivity, selectivity and reproducibility. Overall, the attributes of the herein described sensing approach can be compared to a very limited number of other electrochemical devices, that are still using a conventional three electrode system, making this paper-sustained device the first electrochemical (bio)sensor with potential to become a portable and low-cost diagnostic tool for 3-NT. In general, the incorporation of molecular imprinting technology coupled to electrochemical transduction enabled the fabrication of suitable smart sensors for wide screening approaches.
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