期刊
FOOD CHEMISTRY
卷 331, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.127365
关键词
Salvia miltiorrhiza; Phenolic acids; Antioxidant activity; Antibacterial activity; Potential healthcare materials
资金
- National Key R&D Program of China [2018YFC1706200]
- National Natural Science Fund of China [81522049, 31571735, 31270007]
- Shanghai Science and Technology Committee Project [17JC1404300, 15430502700]
- Zhejiang Provincial Ten-thousand Program for Leading Talents of Science and Technology Innovation [2018R52050]
- Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents [2018-62-3]
- New Century Talent Project [NECT-13-0902]
- Dawn Program of Shanghai Education Commission [16SG38]
- Opening project of Zhejiang provincial preponderant and characteristic subject of Key University (Traditional Chinese Pharmacology), Zhejiang Chinese Medical University [ZYAOX2018019]
Phenolic acids from Salvia miltiorrhiza have been widely used in nutritious, health-promoting products with an increasing demand. In the current study, two biosynthetic genes RAS (rosmarinic acid synthase) and CYP98A14 (a cytochrome P450-dependent monooxygenase) were successfully introduced into S. miltiorrhiza hairy roots. Overexpression of RAS and CYP98A14 resulted in higher content of phenolic acids (up to over 3-fold) in transgenic lines compared to the control. Meanwhile, DPPH results revealed that engineered S. miltiorrhiza hairy roots had stronger antioxidant activities than the control. In addition, phenolic acid crude extracts of the engineered hairy root lines overexpressing RAS or CYP98A14 showed improved antibacterial activities compared to the control lines. Our work exhibits a useful strategy for enhancement of phenolic acid production and bioactivities of S. miltiorrhiza hairy roots by genetic manipulation of RAS and CYP98A14, and also provides a new resource material to obtain active phenolic acids for food and healthy products.
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