期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 103, 期 -, 页码 1327-1333出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2017.05.055
关键词
Ginseng leaves; Polysaccharide; Macrophage; NK-cell; Lung metastasis
资金
- Food Functionality Evaluation program under the Ministry of Agriculture, Food and Rural Affairs
- Korea Food Research Institute
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2014R1A1A3050928]
- National Research Foundation of Korea [2014R1A1A3050928] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, we purified the polysaccharide fraction (GS-P) from the leaves of Panax ginseng C.A. Meyer and analyzed its monosaccharide composition and antitumor and antimetastatic activity in vitro and in vivo. GS-P is a 10.2 kDa pectic polysaccharide consisting of 15 different monosaccharides. GS-P treatment significantly inhibited metastasis in mice, in a dose-dependent manner. GS-P was not cytotoxic to colon 26-M3.1 cells and increased mouse splenocyte proliferation. Secretion of tumor necrosis factor (TNF)-alpha and interleukin (IL)-12 was enhanced in the peritoneal exudate macrophages (PEMs) of GS-P-treated mice. Moreover, PEMs obtained from GS-P-treated mice showed significantly higher tumoricidal activity against colon 26-M3.1 cells, and splenocytes from GS-P-treated mice significantly enhanced NK cell cytotoxicity against YAC-1 tumor cells. Pretreatment with anti-asialo GM1 (an antibody for NK cell depletion) partly suppressed the inhibitory effects of GS-P on lung metastasis. These data suggest that GS-P exhibits antimetastatic activity by promoting the activation of macrophages and NK cells. (C) 2017 Elsevier B.V. All rights reserved.
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