期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 104, 期 -, 页码 1648-1655出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.03.140
关键词
Earthworm; Eisenia fetida; Chitinase; Glycoside hydrolase (GH) family 18
资金
- Grants-in-Aid for Scientific Research [16K08116] Funding Source: KAKEN
Chitin is the second most abundant biopolymer in nature and is an important resource. In this study, we identified a chitinase gene, named Eisenia fetida-Chitinase (EF-Chi) gene, of 1494 base pairs (bp) that encodes a protein of 498 amino acids as indicated by the corresponding mRNA sequence. The amino acid sequence of EF-Chi was similar to those of chitinases from Eisenia andrei (99%), Branchiostoma floridae (50%) and Oryzias latipes (49%), and a gene encoding mature EF-Chi was expressed in the GS115 strain of Pichia pastoris. The molecular mass of the purified recombinant EF-Chi (rEF-Chi) was estimated to be 60 kDa and catalytically important residues of chitinases of the glycoside hydrolase (GH) family 18 were conserved in EF-Chi. The optimal catalytic temperature of rEF-Chi was identified as 60 degrees C, and the hydrolytic product from colloidal chitin was N-acetyl-chitobiose, suggesting that EF-Chi is an exo-type enzyme. (C) 2017 Elsevier B.V. All rights reserved.
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