Partial characterization of glutathione S-transferase (GST) isolated from hepatocytes of Cyprinus carpio from river Ravi Punjab, Pakistan

Glutathione S-transferase (GST) is vital in oxidative stress. Keeping in view the importance of this enzyme, present study was conducted for partial characterization of GST from hepatocytes of Cyprinus carpio. The inferences showed higher (GST) activity in liver of C. carpio collected from Chashma Barrage (144.33 +/- 1.15) as compared to Trimmu Head works (128.66.3 +/- 0.577) and Rasul Barrage (111.66 +/- 0.577) sampling sites. The highest GST activity was 128 +/- 0.333 U mL(-1) from the crude extract fraction of liver as compared to other fractions of appraisal fish liver. Whereas the lowest activity (109 +/- 0.666 U mL(-1)) was from desalted fraction. Optical density was recorded by spectrophotometer at 280 nm, the fractions that showed high optical density were subjected to enzyme assay. Highest specific activity was 183.63, 153.84 and 162.50 U mg(-1) for purified liver GST of C. carpio captured from Trimmu Head works, Rasul Barrage and Chashma Barrage, respectively. Whereas, fold purification was 2.86, 2.69 and 2.10 while, percent recovery was 78.90, 72.07 and 63.19 %, respectively for purified liver GST. The optimum pH was 6.5, 6.0 and 6.8 whereas, temperature was 30.5 degrees C, 31 degrees C and 30 degrees C followed by substrate concentration 40.3 mM, 40 mM and 41 mM, for purified liver GST of C. carpio captured from Trimmu Head works, Rasul Barrage and Chashma Barrage, respectively. It should be noted that detailed studies can be beneficial in the fields of physiology, biochemistry and toxicology.