期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 104, 期 -, 页码 1916-1924出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.04.114
关键词
Blood clot; Chitosan; Mixed leukocyte; Leukotriene B-4
资金
- Canadian Institutes of Health Research [144440-BME, 185810]
- Fonds de la Recherche Quebec Sante [22341]
Controlling the blood clot phenotype in a surgically prepared wound is an evolving concept in scaffold guided tissue engineering. Here, we investigated the effect of added chitosan (80% or 95% Degree of Deacetylation, DDA) or coagulation factors (recombinant human Factor Vila, Tissue Factor, thrombin) on inflammatory factors released by blood clots. We tested the hypothesis that 80% DDA chitosan specifically enhances leukotriene B-4 (LTB4) production. Human or rabbit whole blood was combined with isotonic chitosan solutions, coagulation factors, or lipopolysaccharide, cultured in vitro at 37 degrees C, and after 4 hours the serum was assayed for LTB4 or inflammatory factors. Only 80% DDA chitosan clots produced around 15-fold more LTB4 over other clots including 95% DDA chitosan clots. All serum contained high levels of PDGF-BB and CXCL8. Normal clots produced very low type I cytokines compared to lipopolysaccharide clots, with even lower IL-6 and IL-12 and more CCL3/CCL4 produced by chitosan clots. Coagulation factors had no detectable effect on clot phenotype. Conclusion In blood clots from healthy individuals, 80% DDA chitosan has a unique influence of inducing more LTB4, a potent neutrophil chemoattractant, with similar production of PDGF-BB and CXCL8, and lower type I cytokines, compared to whole blood clots. (C) 2017 Elsevier B.V. All rights reserved.
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