期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 95, 期 -, 页码 1280-1288出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2016.11.025
关键词
Thiothrix fructosivorans; Thiothrix nivea; Sheath; Elongation; Fine structure
资金
- JSPS KAKENHI [JP16K07714]
- Joint Usage/Research Program on Zero-Emission Energy Research at the Institute of Advanced Energy, Kyoto University [ZE28 A-4]
Thiothrix strains are filamentous sulfur-oxidizing bacteria common in activated sludge. Some of the members, including Thiothrix nivea and T. fructosivorans, are known to form a microtubular sheath that covers a line of cells. The sheaths are assemblages of [-> 4)-beta-D-GlcN-(1 -> 4)-beta-D-Glc-(1 ->](n), modified with unusual deoxy sugars. In an attempt to elucidate the sheath-forming mechanism, the patterns of sheath formation and cell proliferation were determined in this study. Prior to analysis, both sheaths were confirmed to be highly de-N-acetylated. Sheaths in viable filaments were N-biotinylated followed by cultivation and then fluorescently immunostained. Epifluorescence microscopy of the filaments revealed ubiquitous elongation of the sheaths. For visualization of the cell proliferation pattern, the cell membrane was fluorescently stained. The epifluorescence images demonstrated that cell proliferation also proceeds ubiquitously, suggesting that sheath elongation proceeds surrounding an elongating cell. In addition, the fine structure of the Thiothrix filaments was analyzed by transmission electron microscopy employing a freeze-substitution technique. The micrographs of freeze-substituted filaments showed that the sheaths were thin and single layered. In contrast, the sheaths in chemically fixed filaments appeared thick and multilayered. Treatment with glutaraldehyde probably caused deformation of the sheaths. Supporting this possibility, the sheaths were found to be deformed or solubilized by N-acetylation. (C) 2016 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据