期刊
FOOD CHEMISTRY
卷 331, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.127305
关键词
Cyanogenic glucosides; Hydrocyanic acid; Method validation; UHPLC-QqQ-MS/MS
资金
- earmarked fund for Key Technologies Research and Development Program from Ministry of Science and Technology of the People's Republic of China [2017YFC1600900]
- Agriculture Research System of China [CARS-11]
- Special Project of Agriculture Produce Quality Safety Risk Assessment from Ministry of Agriculture and Rural Affairs of the People's Republic of China [GJFP2019043]
An ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS) method was established and validated for the simultaneous quantification of eight cyanogenic glucosides (CNGs) in agri-food. The eight CNGs were linamarin, lotaustralin, linustatin, neolinustatin, taxiphyllin, amygdalin, dhurrin and prunasin. CNGs were extracted with aqueous methanol and cleaned via solid-phase extraction. Analytes were separated with a C-18 column via gradient elution. MS/MS analysis was performed with electrospray ionisation in positive mode. Quantification was performed in multiple reaction monitoring mode. Satisfactory validation results were obtained in terms of linearity, sensitivity, precision and accuracy, matrix effect and stability. The method was applied in typical cyanogenic agri-food. CNGs in cassava, linseed, bamboo, sorghum, apricot, almond and lima bean were analyzed.
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