期刊
SMALL METHODS
卷 4, 期 8, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smtd.201900260
关键词
biosensors; ethylene; hormones; reporters; signaling
资金
- NSF IOS [1650139, 1444561, 1750006]
- USDA-AFRI [12428960]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1650139] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1750006] Funding Source: National Science Foundation
Phytohormone ethylene regulates numerous aspects of plant physiology, from fruit ripening to pathogen responses. The molecular basis of ethylene biosynthesis and action has been investigated for over 40 years, and a combination of biochemistry, genetics, cell, and molecular biology have proven successful at uncovering the core machinery of the ethylene pathway. A number of molecular tools have been developed over the years that enable visualization of the sites of ethylene production and response in the plant. Genetically encoded biosensors take advantage of reporter proteins, i.e., fluorescent, luminescent, or colorimetric markers, to highlight the tissues that make, perceive, or respond to the hormone. This review describes the different types of biosensors currently available to the ethylene community and discusses potential new strategies for developing the next generation of genetically encoded ethylene reporters.
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